Design Process
From therapeutic hypothesis to experimental answer.
1. Choose the target and functional site
Define the protein, the biologically important region or interface, and the effect the molecule should produce.
2. Design from sequence
Use the target protein sequence and selected region to computationally design an oligonucleotide binder.
3. Synthesize the defined molecule
Produce the designed oligonucleotide using a reproducible synthetic workflow.
4. Test biological function
Evaluate whether the molecule produces the intended effect in the relevant biochemical, cellular or analytical model.
5. Advance with the partner
Use experimental data to refine the molecule, expand validation and determine the next development step.
At Nemdx, we apply, to a given targeted protein, our process for optimizing RNA-protein binding:
Characterize RNA - protein binding in silico by analyzing the physiochemical properties of interactions between amino acids and nucleotides and identify optimal binding site(s)
